Start With a Focused Pilot.
Expand into Large Enzyme Engineering Projects

EnduraFAST pilot programs are designed to help enzyme teams generate clear, decision-useful stability data before committing to larger screening or engineering work.

Begin with a focused proof-of-concept assessment to confirm EnduraFAST compatibility, evaluate stability under the conditions that matter to your process, and identify whether the platform can support deeper variant ranking, large-library engineering, or scale-up durability characterization.

Bring your enzyme, your stress conditions, and your performance goals. We’ll help define the right starting point.

THE STARTING POINT

First, Prove the Signal Matters.

A focused feasibility pilot can evaluate EnduraFAST with your enzyme, your stress conditions, and the decision your team needs the data to support. From there, the work can expand into high throughput screening including variant ranking to inform variant design and shorten engineering cycle times. We can additionally perform broader stress mapping, time-based durability studies and scale up feasibility studies.

PILOT PATHWAY

One Starting Point. Multiple Ways to Expand.

Every EnduraFAST engagement begins with a focused proof-of-concept assessment. From there, the pilot path can expand based on your enzyme, your conditions, and the decisions you need to make next.

Start Here

Then Expand Based on Your Goal

Engineering Path

Characterization Path

The Goal

Move from an initial signal check to the right next experiment — whether that means ranking variants, engineering stronger candidates, or generating process-ready durability data.

PILOT 1

Proof of Concept & Initial Stability Screening

Start with a focused assessment designed to confirm whether EnduraFAST is the right fit for your enzyme, your conditions, and your next development decision.

This pilot establishes whether your enzyme folds properly and retains activity as an EnduraFAST fusion, then evaluates early stability behavior under the process-relevant conditions that matter to your workflow.

What this pilot is designed to answer

Does your enzyme work as an EnduraFAST fusion?

We confirm that your enzyme folds properly and remains active when paired with EnduraFAST.

Does the signal behave predictably under stress?

We evaluate thermostability and any desired chemical or process stress conditions, such as solvent tolerance, high salinity, pH, or low-oxygen environments.

Is there enough useful data to expand?

The results help determine whether to move into small-library testing, large-library engineering, or scale-up durability characterization.

PILOT 2

Small Library Testing

Once EnduraFAST compatibility is established, the next step is to evaluate a focused set of variants and identify which candidates show the strongest stability signal before committing to broader screening or deeper development work.

This pilot is designed for teams that already have a defined enzyme family, early variant set, or focused engineering direction and want stability data that can help prioritize which candidates are worth advancing.

What this pilot is designed to answer

Which variants hold up best under relevant stress?

Screen a focused variant set under the conditions that matter to your workflow, including thermal, chemical, pH, solvent, salt, or low-oxygen conditions where appropriate.

How should stability data inform candidate ranking?

Use EnduraFAST signal behavior to compare variants and identify candidates with stronger evidence of folding, stability, or durability under challenge conditions.

Which candidates should move forward?

Use the results to prioritize variants for activity confirmation, larger library campaigns, or additional characterization.

PILOT 3

Iterative Large-Library Engineering Campaigns

When the goal is to drive meaningful stability or performance improvement, EnduraFAST can support larger, iterative engineering campaigns built around high-throughput screening.

This pilot is designed to move beyond focused variant ranking and into larger libraries, where each round generates data that informs the next library design. Campaigns typically progress through multiple rounds, with each successive library becoming smaller, more focused, and more aligned with the target stability or performance profile.

What this pilot is designed to answer

Can we identify stronger candidates at library scale?

Screen large variant libraries to find candidates with improved stability signal under the stress conditions that matter to your workflow.

Is the library improving round over round?

Use each screening round to measure progress, narrow the candidate pool, and inform the next round of library design.

Which candidates are ready for confirmation?

Advance enriched, stability-improved variants for activity verification, sequence review, and follow-up testing.

PILOT 4

Scale-Up Stability Assessment

Once promising enzymes or variants have been identified, the next question is how they perform under the conditions that matter for real process use.

This pilot is designed to generate deeper durability data across your best candidates, including half-life, thermal stability, chemical stability, Tm, and time-resolved performance under defined challenge conditions.

What this pilot is designed to answer

How durable are your best candidates under stress?

Evaluate how selected enzymes or variants behave under thermal, chemical, or process-relevant challenge conditions.

Where do stability limits begin to appear?

Measure decay behavior, half-life, thermal stability, chemical stability, and Tm to understand where each candidate starts to lose performance.

Which candidates are ready for downstream development?

Use durability data to support process design, enzyme loading decisions, scale-up planning, and final candidate selection.

WHAT YOU GET FROM A PILOT

Decision-Useful Data, Not Just Another Screen.

EnduraFAST pilots are designed to produce practical data that helps your team decide what to advance, what to deprioritize, and where additional engineering or characterization is justified.

Depending on the pilot path, results may support early compatibility decisions, variant ranking, large-library campaign design, scale-up durability assessment, or downstream process planning.

Pilot outputs may include

Fusion Compatibility Confirmation

Evidence that your enzyme folds properly and retains activity as an EnduraFAST fusion.

Condition-by-Condition Stability Maps

A clear view of how candidates behave across temperature, pH, solvents, salts, oxygen conditions, storage time, or other process-relevant stressors.

Ranked Variant Sets

Prioritized candidates based on stability signal, with activity comparison where included.

Time-Resolved Decay Profiles

Readouts that show how stability changes over time under defined challenge conditions.

Half-Life, Tm, and Durability Metrics

Quantitative data to support process design, enzyme loading decisions, and downstream development.

Recommended Next Steps

A practical interpretation of whether to advance candidates, expand screening, redesign the library, or move into deeper durability characterization.

HOW IT WORKS

Define the Right Pilot Before You Commit.

A pilot conversation starts with the enzyme, the process conditions, and the decision your team needs to make. From there, we help define the smallest useful scope that can produce meaningful data.

READY TO START?

See Whether EnduraFAST Can Support Your Enzyme Workflow.

Bring us your enzyme, your process conditions, and the decision your team needs to make next. We’ll help define the right starting point, pilot scope, and path forward.

Whether you are validating compatibility, ranking a focused variant set, engineering larger libraries, or characterizing durability for scale-up, the first step is a focused conversation.

Share your enzyme, your stress conditions, and your development goals.

We’ll help determine the smallest useful pilot that can generate decision-ready data.